composition alone shows this; besides nitrogen, they contain carbon, hydrogen, oxygen, and sulphur. Being distinguished by a very great instability under many conditions in which other compounds remain unchanged, these substances are fitted for those perpetual changes which form the first condition of vital activity. These complex and changeable nitrogenous substances of the organism are called proteïd substances. The white of eggs is a familiar example of such a substance. They are also contained in the flesh of animals, the curdy elements of milk, the glutinous matter of wheaten flour, or so-called gluten, which forms the chief component of macaroni, &c.
Nitrogen occurs in the earth's crust, in compounds either forming the remains of plants and animals, or derived from the nitrogen of the atmosphere as a consequence of its combination with the other component parts of the air. It is not found in other forms in the earth's crust; so that nitrogen must be considered, in contradistinction to oxygen, as an element which is purely superficial, and does not extend to the depths of the earth.2
Nitrogen is liberated in a free state in the decomposition of the nitrogenous organic substances entering into the composition of organisms—for instance, on their combustion. All organic substances burn when heated to redness with oxygen (or substances readily yielding it, such as oxide of copper); the oxygen combines with the carbon, sulphur, and hydrogen, and the nitrogen is evolved in a free state, because at a high temperature it does not form any stable compound, but remains uncombined. Carbonic anhydride and water are formed from the carbon and hydrogen respectively, and therefore to obtain pure nitrogen it is necessary to remove the carbonic anhydride from the gaseous products obtained. This may be done very easily by the action of alkalis—for instance, caustic soda. The amount of nitrogen in organic substances is determined by a method founded on this.
It is also very easy to obtain nitrogen from air, because oxygen combines with many substances. Either phosphorus or metallic copper is usually employed for removing the oxygen from air, but, naturally, a number of other substances may also be used. If a small saucer on which a piece of phosphorus is laid be placed on a cork floating on water, and the phosphorus be lighted, and the whole covered with a glass bell jar, then the air under the jar will be deprived of its oxygen, and nitrogen only will remain, owing to which, on cooling, the water will rise to a certain extent in the bell jar. The same object (procuring nitrogen from air) is attained much more conveniently and perfectly by passing air through a red-hot tube containing copper filings. At a red heat, metallic copper combines with oxygen and gives a black powder of copper oxide. If the layer of copper be sufficiently long and the current of air slow, all the oxygen will be absorbed, and nitrogen alone will pass from the tube.3
Nitrogen may also be procured from many of its compounds with oxygen and hydrogen , but the best fitted for this purpose is a saline mixture containing, on the one hand, a compound of nitrogen with oxygen, termed nitrous anhydride, N 2 O 3 , and on the other hand, ammonia, NH 3 —that is, a compound of nitrogen with hydrogen. By heating such a mixture, the oxygen of the nitrous anhydride combines with the hydrogen of the ammonia, forming water, and gaseous nitrogen is evolved, 2NH 3 + N 2 O 3 = 3H 2 O + N 4 . Nitrogen is procured by this method in the following manner:—A solution of caustic potash is saturated with nitrous anhydride, by which means potassium nitrite is formed. On the other hand, a solution of hydrochloric acid saturated with ammonia is prepared; a saline substance called sal-ammoniac, NH 4 Cl, is thus formed in the solution. The two solutions thus prepared are mixed together and heated. Reaction takes place according to the equation KNO 2 + NH 4 Cl = KCl + 2H 2 O + N 2 . This reaction proceeds in virtue of the fact that